Selected Publications

Research Articles

  1. S.-J. Lai, I-F. Tu, T.-S. Tseng, Y.-H. Tsai, S.-H. Wu, The deficiency of poly-β-1,6-N-acetyl-glucosamine deacetylase trigger A. baumannii to convert to biofilm-independent colistin-tolerant cells. Sci. Rep. 2023, 13, 2800.
  2. D. Cardella, Y.-H. Tsai, L. Y. P. Luk, Towards the use of an amino acid cleavable linker for solid-phase chemical synthesis of peptides and proteins. Org. Biol. Chem. 2023, 21, 966.
  3. L. A. Spear, Y. Huang, J. Chen, A. R. Nödling, S. Virdee, Y.-H. Tsai, Selective inhibition of cysteine-dependent enzymes by bioorthogonal tethering. J. Mol. Biol. 2022, 434, 167524.
  4. V. L. Barlow, Y.-H. Tsai, Acetylation at lysine 86 of Escherichia coli HUβ modulates the DNA-binding capability of the protein. Front. Microbiol. 2022, 12, 809030.
  5. D. Cardella, W. Deng, L. Y. P. Luk, Y.-H. Tsai, Effect of trimethine cyanine dye- and folate-conjugation on the in vitro biological activity of proapoptotic peptides. Biomolecules 2022, 12, 725.
  6. R. Cruz-Samperio, R. J. Mart, L. Y. P. Luk, Y.-H. Tsai, A. T. Jones, R. K. Allemann, Spatio-temporal photo-activation of cytotoxic proteins. ChemBioChem 2022, 23, e202200115.
  7. E. M. Mills, V. L. Barlow, A. T. Jones, Y.-H. Tsai, Development of mammalian cell logic gates controlled by unnatural amino acids. Cell Rep. Methods 2021, 1, 100073.
  8. T. L. Williams, D. Iskandar, A. R. Nödling, Y. Tan, L. Y. P. Luk, Y.-H. Tsai, Transferability of N-terminal mutations of pyrrolysyl-tRNA synthetase in one species to that in another species on unnatural amino acid incorporation efficiency. Amino Acids 2021, 53, 89.
  9. X. Zheng, Z. Li, W. Gao, X. Meng, X. Li, L. Y. P. Luk, Y. Zhao, Y.-H. Tsai, C. Wu, Condensation of 2-((alkylthio)(aryl)methylene)malononitrile with 1,2-aminothiol as a novel bioorthogonal reaction for site-specific protein modification and peptide cyclization. J. Am. Chem. Soc. 2020, 142, 5097.
  10. T. M. S. Tang, D. Cardella, A. J. Lander, X. Li, J. S. Escudero, Y.-H. Tsai, L. Y. P. Luk, Use of an asparaginyl endopeptidase for chemo-enzymatic peptide and protein labeling. Chem. Sci. 2020, 11, 5881.
  11. A. R. Nödling, E. M. Mills, X. Li, D. Cardella, E. J. Sayers, S.-H. Wu, A. T. Jones, L. Y. P. Luk, Y.-H. Tsai, Cyanine dye mediated mitochondrial targeting enhances the anti-cancer activity of small-molecule cargoes. Chem. Commun. 2020, 56, 4672.
  12. V. L. Barlow, S.-J. Lai, C.-Y. Chen, C.-H. Tsai, S.-H. Wu, Y.-H. Tsai, Effect of membrane-fusion protein AdeT1 on the antimicrobial resistance of Escherichia coli. Sci. Rep. 2020, 10, 20464.
  13. N. G. Eissa, E. J. Sayers, D. Birch, S. G. Patel, Y.-H. Tsai, H. M. Nielsen, A. T. Jones, EJP18 peptide derived from the juxtamembrane domain of epidermal growth factor receptor represents a novel membrane-active cell-penetrating peptide. Biochem. J. 2020, 477, 45. Commented in Biochemical Journal.
  14. S. G. Patel, E. J. Sayers, L. He, R. Narayan, T. L. Williams, E. M. Mills, R. K. Allemann, L. Y. P. Luk, A. T. Jones, Y.-H. Tsai, Cell-penetrating peptide sequence and modification dependent uptake and subcellular distribution of green florescent protein in different cell lines. Sci. Rep. 2019, 9, 6298.
  15. A. R. Nödling, K. Świderek, R. Castillo, J. W. Hall, A. Angelastro, L. C. Morrill, Y. Jin, Y.-H. Tsai, V. Moliner, L. Y. P. Luk, Reactivity and selectivity of iminium organocatalysis improved by a protein host. Angew. Chem. Int. Ed. 2018, 57, 12478. F1000Prime recommended.
  16. T. Suzuki, M. Asami, S. G. Patel, L. Y. P. Luk, Y.-H. Tsai, A. C. F. Perry, Switchable genome editing via genetic code expansion. Sci. Rep. 2018, 8, 10051. News in Science, The Conversation, GenomeWeb and Biotechnology and Biological Sciences Research Council (BBSRC). Press releases from Cardiff University and University of Bath.
  17. K. Świderek, A. R. Nödling, Y.-H. Tsai, L. Y. P. Luk, V. Moliner, Reaction mechanism of organocatalytic Michael addition of nitromethane to cinnamaldehyde: a case study on catalyst regeneration and solvent effects. J. Phys. Chem. A 2018, 122, 451.
  18. T. L. Williams, A. R. Nödling, Y.-H. Tsai, L. Y. P. Luk, Carbapenems as water soluble organocatalysts. Wellcome Open Res. 2018, 3, 107.
  19. J.-H. Liao, C.-H. Tsai, S. G. Patel, J.-T. Yang, I-F. Tu, M. Lo Cicero, M. Lipka-Lloyd, W.-L. Wu, W.-J. Shen, M.-R. Ho, C.-C. Chou, G. R. Sharma, H. Okanishi, L. Y. P. Luk, Y.-H. Tsai, S.-H. Wu, Acetylome of Acinetobacter baumannii SK17 reveals a highly-conserved modification of histone-like protein HU. Front. Mol. Biosci. 2017, 4, 77. News in The Conversation.
  20. S.-J. Lai, I-F. Tu, W.-L. Wu, J.-T. Yang, L. Y. P. Luk, M.-C. Lai, Y.-H. Tsai, S.-H. Wu, Site-specific His/Asp phosphoproteomic analysis of prokaryotes reveals putative targets for drug resistance. BMC Microbiol. 2017, 17, 123.
  21. Y.-H. Tsai, S. Essig, J. R. James, K. Lang, J. W. Chin, Selective, rapid and optically switchable regulation of protein function in live mammalian cells. Nat. Chem. 2015, 7, 554. News in Chemical & Engineering News. Highlighted in Nature Methods and ACS Chemical Biology.
  22. S. Götze, A. Reinhardt, A. Geißner, N. Azzouz, Y.-H. Tsai, R. Kurucz, D. Varon Silva, P. H. Seeberger, Investigation of the protective properties of glycosylphosphatidylinositol-based vaccine candidates in a Toxoplasma gondii mouse challenge model. Glycobiology 2015, 25, 984.
  23. S. Götze, N. Azzouz, Y.-H. Tsai, U. Groß, A. Reinhardt, C. Anish, P. H. Seeberger, D. Varon Silva, Diagnosis of toxoplasmosis using a synthetic glycosylphosphatidylinositol glycan. Angew. Chem. Int. Ed. 2014, 53, 13701.
  24. Y.-H. Tsai, S. Götze, I. Vilotijevic, M. Grube, D. Varon Silva, P. H. Seeberger, A general and convergent synthesis of diverse glycosylphosphatidylinositol glycolipids. Chem. Sci. 2013, 4, 468.
  25. Y.-H. Tsai, S. Götze, N. Azzouz, H. S. Hahm, P. H. Seeberger, D. Varon Silva, A general method for synthesis of GPI anchors illustrated by the total synthesis of the low molecular weight antigen from Toxoplasma gondii. Angew. Chem. Int. Ed. 2011, 50, 9961.
  26. M. Oberli, M. Tamborrini, Y.-H. Tsai, D. B. Werz, T. Horlacher, A. Adibekian, D. Gauss, H. M. Möller, G. Pluschke, P. H. Seeberger, Molecular analysis of carbohydrate-antibody interactions: case study using a Bacillus anthracis tetrasaccharide. J. Am. Chem. Soc. 2010, 132, 10239.
  27. R. Kikkeri, X. Liu, A. Adibekian, Y.-H. Tsai, P. H. Seeberger, Facile synthesis of size dependent Ru(II)–carbohydrate dendrimers via click chemistry. Chem. Commun. 2010, 46, 2197.
  28. M.-L. Hecht, Y.-H. Tsai, X. Liu, C. Wolfrum, P. H. Seeberger, Synthetic inositol phosphoglycans related to GPI lack insulin-mimetic activity. ACS Chem. Biol. 2010, 5, 1075.
  29. Y.-L. Yang, F.-L. Yang, Z.-Y. Huang, Y.-H. Tsai, W. Zou, S.-H. Wu, Structural variation of glycolipids from Meiothermus taiwanensis ATCC BAA-400 under different growth temperatures. Org. Biomol. Chem. 2010, 8, 4252.
  30. C.-T. Ren, Y.-H. Tsai, Y.-L. Yang, W. Zou, S.-H. Wu, Synthesis of a tetrasaccharide glycosyl glycerol. Precursor to glycolipids of Meiothermus taiwanensis ATCC BAA-400. J. Org. Chem. 2007, 72, 5427.


  1. J. Chen, Y.-H. Tsai*, Applications of genetic code expansion in studying protein post-translational modification. J. Mol. Biol. 2022, 434, 167424.
  2. Y.-H. Tsai, T. Doura, S. Kiyonaka, Tethering-based chemogenetic approaches for the modulation of protein function in live cells. Chem. Soc. Rev. 2021, 50, 7909.
  3. H. C. Hayes, L. Y. P. Luk, Y.-H. Tsai, Approaches for peptide and protein cyclisation. Org. Biomol. Chem. 2021, 19, 3983.
  4. E. M. Mills, V. L. Barlow, L. Y. P. Luk, Y.-H. Tsai, Applying switchable Cas9 variants to in vivo gene editing for therapeutic applications. Cell Biol. Toxicol. 2020, 36, 17.
  5. A. R. Nödling, N. Santi, T. L. Williams, Y.-H. Tsai, L. Y. P. Luk, Enabling protein-hosted organocatalytic transformations. RSC Adv. 2020, 10, 16147.
  6. A. R. Nödling, L. A. Spear, T. L. Williams, L. Y. P. Luk, Y.-H. Tsai, Using genetically incorporated unnatural amino acids to control protein functions in mammalian cells. Essays Biochem. 2019, 63, 237.
  7. Y.-H. Tsai, X. Liu, P. H. Seeberger, Chemical biology of glycosylphosphatidylinositol anchors. Angew. Chem. Int. Ed. 2012, 51, 11438.
  8. Y.-H. Tsai, M. Grube, P. H. Seeberger, D. Varon Silva, Glycosylphosphatidylinositols of protozoan parasites. Trends Glycosci. Glycotechnol. 2012, 24, 231.


  1. Y.-H. Tsai, H. Iwaï, K. Pors, Editorial: chemical biology tools for peptide and protein research, Front. Chem. 2022, 10, 861699.

Book Chapter

  1. E. J. Sayers, V. L. Barlow, Y.-H. Tsai, A. T. Jones. (2022) Quantitative subcellular analysis of cyclic cell-penetrating peptide EJP18 in nonadherent cells. In: Langel Ü. (eds) Cell Penetrating Peptides. Methods in Molecular Biology, vol 2383. Humana, New York, NY.


  1. N. Azzouz, S. Götze, P. H. Seeberger, D. Varon Silva, Y.-H. Tsai, Synthesis of diverse glycosylphosphatidylinositol glycans from Toxoplasma gondii and their application as vaccines and diagnostics. European Patent EP2890703 B8, filed 23/07/2013; US Patent US20150203523 B2, filed 23/07/2013.

Patent Applications

  1. N. Azzouz, S. Götze, P. H. Seeberger, D. Varon Silva, Y.-H. Tsai, Synthesis of diverse glycosylphosphatidylinositol glycans from Toxoplasma gondii and their application as vaccines and diagnostics. European Patent Application EP2690104 A1, filed 24/07/2012; World Patent Application WO2014016317 A1, filed 23/07/2013.
  2. N. Azzouz, S. Götze, P. H. Seeberger, D. Varon Silva, Y.-H. Tsai, Synthesis of diverse glycosylphosphatidylinositol glycans and glycolipids from Toxoplasma gondii and their application as diagnostic markers and vaccines. European Patent Application EP2690438 A1, filed 24/07/2012.